Info-enriched characterization of cell populations. Morphological information from photographs can increase into the richness of flow cytometry data. By way of example, the figure exhibits an in any other case common apoptosis assay employing Annexin V and PI, introducing cell imaging to characterize cells in Each individual inhabitants to reveal morphologically distinctive attributes. These insights couldn't have been attained from multiplex staining by yourself.
As samples are obtained around the Attune CytPix Flow Cytometer, the large-speed brightfield digital camera captures and shops photographs of detected events in a rate around 6,000 visuals for every 2nd, dependant upon the flow amount and impression dimensions. For greater overall flexibility, Attune Cytometric Software program lets you change the impression capture frequency as wanted and select a particular gate for picture seize, if essential.
Incorporate acoustic focusing know-how with substantial-parameter Examination for adaptable spectral unmixing and traditional workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic focusing which has a significant-speed brightfield digital camera for simultaneous significant throughput flow cytometry and higher resolution brightfield imaging.
"A picture is just not pretty really worth one thousand dots, but it really truly helps to see pictures. It is great to discover photos that correlate with staining. As an example, AnnexinV-good cells which can be morphologically different than healthful cells, or CD14+ monocytes which might be greater plus much more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it's illuminating to determine the amount of doublets slip through standard singlet gates, and great to confirm the position of unpredicted double-optimistic situations in my Evaluation."
Pair your Attune flow cytometer having an autosampler for improved workflow performance, and increase robotic integration for max throughput.
Our researchers will design panels for you as over and over you'll need—totally free, no product acquire necessary.
The Attune NxT cytometer is the trustworthy workhorse instrument your lab can rely upon for consistent and trustworthy overall performance. The Attune CytPix model incorporates a significant-velocity brightfield camera and AI-driven graphic Investigation computer software, with the latest updates which include device learning resources and an intuitive person interface, enabling Increased knowledge precision, precision, and more rapidly insights into sample biology.
Potentially most significantly, these activities have cells of Plainly distinctive phenotypes which could lead to incorrect conclusions pertaining to double positive activities (specifically in rare populations).
Seem waves help specific mobile alignment, resulting in Outstanding details quality and ~10X more rapidly sample flow fees.
Application Notice: Automated image Assessment minimizes user-to-person variability in flow cytometry gating procedures
Leverage the power of Innovative technology and innovation to speed up your discoveries. Rely on in the Attune Flow Cytometer family members to provide the dependability, speed, and precision that set your function aside.
Adaptable payment plan from Agilent Economic Remedies help you get the significant instrumentation you may need for rapid job, or to grow your lab.
Gating on these prolonged parameters lets you rapidly and accurately detect populations of fascination and make sure gating technique with little if any guide overview. The Attune program provides usage of condition-of-the-artwork machine Mastering Assessment resources in just an expertly intended consumer interface, rendering it extremely convenient to use.
The Attune Xenith cytometer is often a higher-parameter spectral analyzer, delivering the get more info two spectral unmixing and standard flow capabilities for complete and adaptable mobile Evaluation.
Agilent Price Guarantee ensures you no less than a decade of instrument use from your day you purchase. Or else, We are going to credit you While using the residual value of the system toward an upgraded design.
What appeared to be a homogeneous population is in fact far more various—an Perception that needs to be regarded as when interpreting effects.